|
KMID : 0525720190240020090
|
|
Journal of Chitin and Chitosan
2019 Volume.24 No. 2 p.90 ~ p.98
|
|
|
Inhibition Effect of a Hot Water Extracts from Sargassum Fulvellum AgainstGluten-induced Inflammation and Oxidative Damage in Human Keratinocytes
|
|
Shin Eun-Ji
Han Eui-Jeong
Kim Min-Ju
Jung Jae-Kyu
Park Jun-Young
Ahn Gin-Nae
|
|
|
Abstract
|
|
|
|
In this study, we investigated the beneficial capacity of Sargassum Fulvellum (S. fulvellum, SF) against gluten-induced inflammation and oxidative damage in HaCaT cells, a human keratinocytes. First of all, we prepared the hot water extract from SF (SFH) and it was used for this study. The result showed SFH has no cytoxicities at the used all concentrations. Also, SFH improved the cell viability by reducing the generation of intracellular reactive oxygen species (ROS) and nitric oxide (NO), and the apoptotic body formation in gluten-stimulated HaCaT cells. In addition, SFH inhibited the expression levels of inflammatory cytokines such as interleukin (IL)-1β, IL-4, IL-5, IL-6, IL-8 and IL-13 compared to the only gluten-stimulated cells. Moreover, western blot analysis revealed that SFH suppressed the activation of nuclear factor (NF)-κB signaling by regulating the phosphorylation and degradation of IκB-α and cytoplasmic NF-κB p65 as well as the translocation of NF-κB p65 into the nucleus. Furthermore, SFH inhibited the activation of extracellular signal regulated kinase (ERK)/P38 signaling in the gluten-stimulated HaCaT cells. Therefore, these results suggest that SFH improved the inflammation and oxidative damage caused by caused by the exposure of gluten in HaCaT cells and it might be a natural material for the improvement of gluten-caused various diseases.
|
|
|
KEYWORD
|
|
|
Sargassum Fulvellum, Gluten, Anti-inflammation, Oxidative stress, HaCaT cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|